dCAS9-VP64_GFP (Plasmid #61422)
|Item||Catalog #||Description||Quantity||Price (USD)|
Standard format: Plasmid sent in bacteria as agar stab
|Concentrated Lentiviral Prep||61422-LVC||
Virus (50µL at titer ≥ 2.5×10⁶ TU/mL)
This material is available to academics and nonprofits only.
Mammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Synthetic; S. pyogenes
D10A and H840A in Cas9
5′ cloning site
3′ cloning site
5′ sequencing primer
GTT TGG ATC TTG GTT CAT TCT CAA GCC TCA G
3′ sequencing primer
- Not Available to Industry
For additional information, protocols and an activator sgRNA design tool, visit our website: http://sam.genome-engineering.org/
Information for Concentrated Lentiviral Prep (Catalog # 61422-LVC)
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Ready-to-use Concentrated Lentiviral Prep particles produced from dCAS9-VP64_GFP (#61422). In addition to the viral particles, you will also receive purified dCAS9-VP64_GFP plasmid DNA.
$150 USD for preparation of 50 µL virus + $30 USD for plasmid.
Store at -80℃. Thaw just before use and keep on ice.
Viral particles are shipped frozen on dry ice.
Plasmid DNA (≥ 200ng) will also be included
in the shipment.
Viral Production & Use
psPAX2 (plasmid #12260)
pMD2.G (plasmid #12259)
Lentivirus was harvested from cell culture medium (DMEM + 10% FBS). Lentiviral particles were then collected by precipitation in polyethylene glycol (PEG) followed by centrifugation. Precipitated pellets containing viral particles were resuspended in PBS.
Requestor is responsible for compliance with
their institution’s biosafety regulations.
Lentivirus is generally considered BSL-2. AAV is
generally considered BSL-1, but may require
BSL-2 handling depending on the insert.
- Not Available to Industry
Viral Quality Control
- Direct fluorescence: Lenti-X cells were transduced with serial dilutions of 61422-LVC. 96 hours later, GFP-positive cells were counted. Read our fluorescence titering assay protocol here.
- Quality control was performed on this lentiviral preparation prior to concentration. Results and explanations of quality control methods can be seen in the Information for Lentiviral Prep section on this page.
Visit our viral production page for more information.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the
Principal Investigator, cite the article in which the plasmids were described,
and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:
was a gift from
(Addgene plasmid # 61422 ; http://n2t.net/addgene:61422 ; RRID:Addgene_61422)
For viral preps, please replace (Addgene plasmid # 61422)
in the above sentence with:
(Addgene viral prep # 61422-LVC)
For your References section:
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.
Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F.
Nature. 2014 Dec 10. doi: 10.1038/nature14136.