Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632 | Protocol

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Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632

DOI: 10.3791/62978

章节

0:00Introduction

0:49Gingival Tissue Pretreatment

1:13Digestion of Gingival Tissue

2:46Cell Passage

3:51Representative Results

6:18Conclusion

Summary


November 6th, 2021

Here we present a modified method for the isolation and culture of human gingival epithelial cells by adding the Rock inhibitor, Y-27632, to the traditional method. This method is easier, less time-consuming, enhances stem cell properties, and produces larger numbers of high-potential epithelial cells both for the laboratory and for clinical applications.

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